This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:
"CRSPR-Cas is a powerful gene editing tool for the therapy of human diseases But its potential for fighting human disease remains untapped Part of the problem is that different CRISPR-Cas systems use different PAM sequences to activate their editing functions and how to directly identify functional PAM sequences in human cells is still an open question Now, researchers have developed PAM Definition by Observable Sequence Excision (PAM-DOSE), a technique for identifying these secret genetic phrases It starts with using bacteria to generate a large library of random sequences on circular strands of DNA Each random sequence is flanked by tdTomato and EGFP to express red and green fluorescent proteins when implanted in a cell Only the correct sequences activate the corresponding CRISPR-Cas pair, whose function is to remove the red-protein portion So a green-only signal indicates a right answer Next-generation sequencing then reveals the details of the correct PAM sequence In a proof-of-principle experiment, the se.."
The rest of the transcript, along with a link to the research itself, is available on the resource itself.
- Subject:
- Biology
- Life Science
- Material Type:
- Diagram/Illustration
- Reading
- Provider:
- Research Square
- Provider Set:
- Video Bytes
- Date Added:
- 12/23/2019